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barcoded primer set  (Illumina Inc)


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    Structured Review

    Illumina Inc barcoded primer set
    Barcoded Primer Set, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcoded+primer+set/barcoded+primer+set/pmc12178574-148-13-19
    Average 90 stars, based on 1 article reviews
    barcoded primer set - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Active layer and permafrost microbial community coalescence increases soil activity and diversity in mixed communities compared to permafrost alone
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA encoding gene were created using a barcoded primer set adapted for the Illumina MiSeq ( ). .. Each 25 μL PCR reaction contained 9.5 μL Certified DNA-Free water, 12.5 μL of QuantaBio’s AccuStart II PCR ToughMix (2x concentration, 1x final), 1 μL Golay barcode tagged Forward Primer (5 μM concentration, 200 pM final), 1 μL Reverse Primer (5 μM concentration, 200 pM final), and 1 μL of template DNA.

    Article Title: ELEXACAFTOR-TEZACAFTOR-IVACAFTOR DECREASES PSEUDOMONAS ABUNDANCE IN THE SINONASAL MICROBIOME IN CYSTIC FIBROSIS
    Article Snippet: Samples were stored and shipped at −20 C or lower, and DNA extractions were done using PowerSoil kits (Qiagen, Germantown, MD). .. PCR amplicon libraries targeting the V4 region of the 16S rRNA encoding gene present in microbial DNA was produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq (San Diego, CA) 23 . .. DNA sequence data was then generated using Illumina paired-end sequencing.

    Article Title: Mapping pesticide-induced metabolic alterations in human gut bacteria.
    Article Snippet: .. Briefly, PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA are produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq. .. Specifically, the V4 region of the 16S rRNA gene (515F-806R) is PCR amplified with region-specific primers that include sequencer adapter sequences used in the Illumina flowcell.

    Article Title: Prebiotic proanthocyanidins inhibit bile reflux–induced esophageal adenocarcinoma through reshaping the gut microbiome and esophageal metabolome
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA–encoding genes present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, Illinois, USA) as previously described ( ).

    Article Title: Prebiotic proanthocyanidins inhibit bile reflux-induced esophageal adenocarcinoma through reshaping the gut microbiome and esophageal metabolome.
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA–encoding genes present in metagenomic DNA were produced using a barcoded primer set adapted 1 8 R E S E A R C H A R T I C L E JCI Insight 2024;9(6):e168112 https://doi.org/10.1172/jci.insight.168112 for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, Illinois, USA) as previously described (82).

    Article Title: Longitudinal effects of oral administration of antimicrobial drugs on fecal microbiota of horses
    Article Snippet: Fecal bacterial DNA was extracted using an automated bead‐based KingFisher extraction and purification system (ThermoFisher Scientific, Waltham, Massachusetts). .. PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired‐end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory.

    Article Title: A Comparison of Three Artificial Rumen Systems for Rumen Microbiome Modeling
    Article Snippet: .. Briefly, PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq [25]. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory.

    Article Title: Changes in permafrost bacterial community composition after thaw across multiple Alaskan locations
    Article Snippet: .. Polymerase chain reaction (PCR) amplicon libraries targeting the 16S rRNA gene present in genomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq (Caporaso et al., 2012), followed by the generation of DNA sequence data using Illumina paired-end sequencing. .. Specifically, the V4 region of the 16S rRNA gene was PCR amplified with region-specific primers (515F/806R) that include sequencer adapter sequences used in the Illumina flowcell (Caporaso et al., 2011, 2012).

    Amplification:

    Article Title: Active layer and permafrost microbial community coalescence increases soil activity and diversity in mixed communities compared to permafrost alone
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA encoding gene were created using a barcoded primer set adapted for the Illumina MiSeq ( ). .. Each 25 μL PCR reaction contained 9.5 μL Certified DNA-Free water, 12.5 μL of QuantaBio’s AccuStart II PCR ToughMix (2x concentration, 1x final), 1 μL Golay barcode tagged Forward Primer (5 μM concentration, 200 pM final), 1 μL Reverse Primer (5 μM concentration, 200 pM final), and 1 μL of template DNA.

    Article Title: ELEXACAFTOR-TEZACAFTOR-IVACAFTOR DECREASES PSEUDOMONAS ABUNDANCE IN THE SINONASAL MICROBIOME IN CYSTIC FIBROSIS
    Article Snippet: Samples were stored and shipped at −20 C or lower, and DNA extractions were done using PowerSoil kits (Qiagen, Germantown, MD). .. PCR amplicon libraries targeting the V4 region of the 16S rRNA encoding gene present in microbial DNA was produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq (San Diego, CA) 23 . .. DNA sequence data was then generated using Illumina paired-end sequencing.

    Article Title: Mapping pesticide-induced metabolic alterations in human gut bacteria.
    Article Snippet: .. Briefly, PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA are produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq. .. Specifically, the V4 region of the 16S rRNA gene (515F-806R) is PCR amplified with region-specific primers that include sequencer adapter sequences used in the Illumina flowcell.

    Article Title: Prebiotic proanthocyanidins inhibit bile reflux–induced esophageal adenocarcinoma through reshaping the gut microbiome and esophageal metabolome
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA–encoding genes present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, Illinois, USA) as previously described ( ).

    Article Title: Prebiotic proanthocyanidins inhibit bile reflux-induced esophageal adenocarcinoma through reshaping the gut microbiome and esophageal metabolome.
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA–encoding genes present in metagenomic DNA were produced using a barcoded primer set adapted 1 8 R E S E A R C H A R T I C L E JCI Insight 2024;9(6):e168112 https://doi.org/10.1172/jci.insight.168112 for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, Illinois, USA) as previously described (82).

    Article Title: Longitudinal effects of oral administration of antimicrobial drugs on fecal microbiota of horses
    Article Snippet: Fecal bacterial DNA was extracted using an automated bead‐based KingFisher extraction and purification system (ThermoFisher Scientific, Waltham, Massachusetts). .. PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired‐end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory.

    Article Title: A Comparison of Three Artificial Rumen Systems for Rumen Microbiome Modeling
    Article Snippet: .. Briefly, PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq [25]. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory.

    Article Title: Changes in permafrost bacterial community composition after thaw across multiple Alaskan locations
    Article Snippet: .. Polymerase chain reaction (PCR) amplicon libraries targeting the 16S rRNA gene present in genomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq (Caporaso et al., 2012), followed by the generation of DNA sequence data using Illumina paired-end sequencing. .. Specifically, the V4 region of the 16S rRNA gene was PCR amplified with region-specific primers (515F/806R) that include sequencer adapter sequences used in the Illumina flowcell (Caporaso et al., 2011, 2012).

    Produced:

    Article Title: ELEXACAFTOR-TEZACAFTOR-IVACAFTOR DECREASES PSEUDOMONAS ABUNDANCE IN THE SINONASAL MICROBIOME IN CYSTIC FIBROSIS
    Article Snippet: Samples were stored and shipped at −20 C or lower, and DNA extractions were done using PowerSoil kits (Qiagen, Germantown, MD). .. PCR amplicon libraries targeting the V4 region of the 16S rRNA encoding gene present in microbial DNA was produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq (San Diego, CA) 23 . .. DNA sequence data was then generated using Illumina paired-end sequencing.

    Article Title: Mapping pesticide-induced metabolic alterations in human gut bacteria.
    Article Snippet: .. Briefly, PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA are produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq. .. Specifically, the V4 region of the 16S rRNA gene (515F-806R) is PCR amplified with region-specific primers that include sequencer adapter sequences used in the Illumina flowcell.

    Article Title: Prebiotic proanthocyanidins inhibit bile reflux–induced esophageal adenocarcinoma through reshaping the gut microbiome and esophageal metabolome
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA–encoding genes present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, Illinois, USA) as previously described ( ).

    Article Title: Prebiotic proanthocyanidins inhibit bile reflux-induced esophageal adenocarcinoma through reshaping the gut microbiome and esophageal metabolome.
    Article Snippet: .. PCR amplicon libraries targeting the 16S rRNA–encoding genes present in metagenomic DNA were produced using a barcoded primer set adapted 1 8 R E S E A R C H A R T I C L E JCI Insight 2024;9(6):e168112 https://doi.org/10.1172/jci.insight.168112 for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, Illinois, USA) as previously described (82).

    Article Title: Longitudinal effects of oral administration of antimicrobial drugs on fecal microbiota of horses
    Article Snippet: Fecal bacterial DNA was extracted using an automated bead‐based KingFisher extraction and purification system (ThermoFisher Scientific, Waltham, Massachusetts). .. PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq. .. DNA sequence data were generated using Illumina paired‐end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory.

    Article Title: A Comparison of Three Artificial Rumen Systems for Rumen Microbiome Modeling
    Article Snippet: .. Briefly, PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA were produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq [25]. .. DNA sequence data were generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory.

    Article Title: Changes in permafrost bacterial community composition after thaw across multiple Alaskan locations
    Article Snippet: .. Polymerase chain reaction (PCR) amplicon libraries targeting the 16S rRNA gene present in genomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq (Caporaso et al., 2012), followed by the generation of DNA sequence data using Illumina paired-end sequencing. .. Specifically, the V4 region of the 16S rRNA gene was PCR amplified with region-specific primers (515F/806R) that include sequencer adapter sequences used in the Illumina flowcell (Caporaso et al., 2011, 2012).

    Sequencing:

    Article Title: Changes in permafrost bacterial community composition after thaw across multiple Alaskan locations
    Article Snippet: .. Polymerase chain reaction (PCR) amplicon libraries targeting the 16S rRNA gene present in genomic DNA were produced using a barcoded primer set adapted for the Illumina MiSeq (Caporaso et al., 2012), followed by the generation of DNA sequence data using Illumina paired-end sequencing. .. Specifically, the V4 region of the 16S rRNA gene was PCR amplified with region-specific primers (515F/806R) that include sequencer adapter sequences used in the Illumina flowcell (Caporaso et al., 2011, 2012).



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